Background Lengthy noncoding RNAs (lncRNAs) are known as key regulators in many cancer types, but their biological functions in nasopharyngeal carcinoma (NPC) remain largely unknown. metastasis. Our biological experiments indicated that ZNRD1-AS1 knockdown reduces NPC cell invasion and metastasis. Further analyses revealed that ZNRD1-AS1 as a ceRNA promotes the migration and invasion of NPC cells by sponging miR-335. We provided evidence that ZNRD1-AS1 facilitates the invasion and metastasis of NPC cells via the miR-335CROCK1 axis. Conclusion Our data shed light on the oncogenic role of ZNRD1-AS1 in NPC tumor development, and a promising therapeutic target for NPC was identified. values of 0.05 and 0.01. Result ZNRD1-AS1 Is Overexpressed in NPC Tissues and Cells In the present study, we first detected the expression of ZNRD1-AS1 in five normal nasopharyngeal and 40 NPC tissue samples through qRT-PCR. Figure 1A reveals that ZNRD1-AS1 expression is higher in the NPC tissues than in the normal nasopharyngeal tissues. We then explored the association between ZNRD1-AS1 expression and the clinicopathological features of the NPC patients (Table 1). The outcomes indicate that ZNRD1-AS1 overexpression can be favorably correlated with undesirable TNM stage and the current presence of lymph node metastasis (Shape 1B, ?,D,D, and ?andF).F). No apparent correlation with major tumor size was noticed (Shape 1C and ?andE).E). Finally, we measured the expression of ZNRD1-While1 in NPC and NP69 cells. The info reveal that ZNRD1-AS1 can be expressed more thoroughly in the NPC cells than in the UR 1102 NP69 cells (Shape 2A). In conclusion, the full total effects imply ZNRD1-AS1 performs a significant role in NPC CBFA2T1 tumorigenesis. Table 1 Romantic relationship Between ZNRD1-AS1 Manifestation with Clinical Features of NPC Individuals 0.05, ** 0.01. Open up in another window Shape 2 ZNRD1-AS1 knockdown demonstrated no obvious results on cell proliferation. (A) The manifestation of ZNRD1-AS1 in NPC and NP69 cells was recognized by qRT-PCR. (B) The manifestation of ZNRD1-AS1 in NPC cells was examined by qRT-PCR after transfection with si-lncRNA and si-NC. (C and D) CCK8 assays demonstrated that ZNRD1-While1 knockdown does not have any obvious results on cell proliferation. (E) Colony development analyses indicated that ZNRD1-AS1 knockdown does UR 1102 not have any obvious results on cell UR 1102 viability. ** 0.01. ZNRD1-AS1 Knockdown Reduces the Invasion and Metastasis of NPC Cells in vitro and in vivo We examined the consequences of particular siRNAs against ZNRD1-AS1 in 5C8F and SUNE1 cells, which display high endogenous ZNRD1-AS1 amounts, to judge the biological part of ZNRD1-While1 in NPC development comprehensively. Our outcomes indicate that siRNA certainly decreases ZNRD1-AS1 manifestation in the NPC cells (Shape 2B). Colony and CCK8 development assay were found in exploring the consequences of ZNRD1-While1 knockdown on cell development. The outcomes indicate that ZNRD1-AS1 knockdown does not have any obvious results on cell proliferation weighed against the control (Shape 2CCE). The wound curing assay result demonstrates that ZNRD1-AS1 knockdown considerably represses the migration of NPC cells (Shape 3A). The transwell invasion assay outcomes display that ZNRD1-AS1 downregulation decreases the invasion of NPC cells (Shape 3B). Based on the founded spontaneous lymph node metastasis model, ZNRD1-AS1 knockdown inhibits tumor axillary lymph node metastasis in vivo (Shape 4ACE). In conclusion, our data reveal that ZNRD1-AS1 knockdown reduces the invasion and metastasis of NPC cells. Open in a separate window Figure 3 ZNRD1-AS1 knockdown reduced cell invasion and metastasis in vitro. (A) Wound healing assays showed that ZNRD1-AS1 knockdown inhibits the migration of NPC cells. (B) Transwell analyses indicated that ZNRD1-AS1 knockdown suppresses the invasion of NPC cells. ** 0.01. Open in a separate window Figure 4 ZNRD1-AS1 knockdown reduced cell invasion and metastasis in vivo. (A) The pictures of nude mice primary tumor sizes in si-NC or si-lncRNA control groups. (B) Image of xenograft tumors in si-NC or si-lncRNA control groups of nude mice. Red arrows indicated popliteal lymph node metastasis. (C) The incidences of popliteal lymph node metastasis of each group were counted. (D) The weights of tumors were detected. (E) The volumes of tumors were detected. ZNRD1-AS1 Is the Direct Target of miR-335 lncRNAs can exert sponge-like effects on various miRNAs and block their regulatory functions on target mRNAs.14C16 Thus, we supposed that ZNRD1-AS1 exerts its function by interacting with some miRNAs. The online bioinformatics database DIANA (http://diana.imis.athena-innovation) predicts that miR-335 contains the putative binding sites of ZNRD1-AS1. We quantified miR-335 expression in NPC cells treated with siRNA against ZNRD1-AS1..