5 Metastases of squamous cell carcinoma, heart, loggerhead turtle (Caretta caretta) No. major normal epithelia and organs from two reptile varieties [the bearded dragon (not done due to lack of samples not relevant (the species does not have this organ) aNumber of instances for each individual organ or cells (#1a# 2b
Cutaneous SCC nests?Basal layer++++++++++++++++?Suprabasal (intermediate) coating++++++++++++++++?Keratin pearls (cornified coating)+++CCCCCInvasive neoplastic cords (dermis)++++++++++++Metastases?Myocardium+++++/?c+++C?Lung+++++/?d+++C?Kidney+++++/?d+++C?Spleen+++C?Liver+++C?Muscle cells+++C Open in a separate windows Reactivity was judged while intense (+++), moderate (++), weak (+), negative (?), or variable (poor/bad) (+/?), respectively aWell-differentiated metastatic cutaneous SCC bPoorly differentiated metastatic cutaneous SCC cOnly the biggest metastatic foci showed immunoreactivity (limited to suprabasal and pre-cornified layers) dReactivity was recognized in a few metastases showing stratified squamous differentiation Open in a separate windows Fig. 3 Squamous cell carcinoma, pores and skin, bearded dragon (Pogona vitticeps). a Immunohistochemistry for acidic alpha-keratin (AE1 positive) showing intense immunoreactivity in all layers of the nests of neoplastic cells, including the cornified coating of the keratin pearls. Pub?=?100 m. b Immunohistochemistry for fundamental alpha-keratin (AE3 positive) showing poor immunoreactivity in the basal and intermediate layers of the nests of neoplastic cells (arrows); no reactivity was observed in the cornified coating of the keratin pearls (*). Pub?=?60 m. Dako EnVision+ System, Harris haematoxylin counterstain In the cutaneous SCCs of both sea turtles, acidic alpha-keratin (AE1 positive) was recognized in the basal and suprabasal layers, and in all of the invasive neoplastic cords (Table ?(Table2,2, Fig.?4a); probably the most intense reactivity using Acetylcorynoline the AE3 antibody was observed in the invasive neoplastic cords recognized in the dermis (Fig. ?(Fig.4b).4b). Adjacent areas of normal epidermis showed a similar pattern to that reported for the epidermis in the study on normal tissues. Open in a separate windows Fig. 4 Squamous cell carcinoma, pores and skin, loggerhead sea turtle (Caretta caretta) No. 1. a Immunohistochemistry for acidic alpha-keratin (AE1 positive) showing intense immunoreactivity in the basal and suprabasal cells, and in all of the invasive neoplastic cords. Pub?=?80 m. b Immunohistochemistry for fundamental alpha-keratin (AE3 positive) showing intense immunoreactivity in the invasive neoplastic cords recognized in the dermis. Pub?=?65 m. Dako EnVision+ System, Harris haematoxylin counterstain Acidic alpha-keratin (AE1 positive) was recognized in all of the metastases in the myocardium from both turtles (Fig.?5a), whereas fundamental alpha-keratin (AE3 positive) was only detected in a few metastases in the myocardium of the 1st turtle. These few metastases were the biggest metastatic foci, and immunoreactivity was limited to suprabasal and pre-cornified layers (Fig. ?(Fig.5b).5b). Similarly, Acetylcorynoline acidic alpha-keratin (AE1 positive) was recognized in all the lung metastases from both turtles (Fig.?6a). Basic alpha-keratin (AE3 positive) was detected in a few metastases in the first turtles lung, mainly in some foci showing stratified squamous differentiation (Fig. ?(Fig.6b).6b). A similar pattern was detected in the kidney metastases. Open in a separate window Fig. 5 Metastases of squamous cell carcinoma, heart, loggerhead turtle (Caretta caretta) No. 2. a Immunohistochemistry for acidic alpha-keratin (AE1 positive) showing intense immunoreactivity in all cells of the metastatic Mouse monoclonal to FUK foci. Bar?=?80 m. b Immunohistochemistry for basic alpha-keratin (AE3 positive) showing intense immunoreactivity only in the suprabasal and pre-cornified layers of a big metastatic focus. Bar?=?110 m. Dako EnVision+ System, Harris haematoxylin counterstain Open in a separate window Fig. 6 Metastases of squamous cell carcinoma, lung, loggerhead turtle (Caretta caretta) No. 1. a Immunohistochemistry for acidic alpha-keratin (AE1 positive) showing strong immunoreactivity in all cells of the metastatic foci; note also the labelling of the normal epithelium of the bronchioles (*). Bar?=?80 m. b Immunohistochemistry for basic alpha-keratin (AE3 positive) in a small metastatic focus showing stratified squamous differentiation (arrows). Bar?=?110 m. Dako EnVision+ System, Harris haematoxylin counterstain All of the small metastatic foci in the spleen from the second turtle showed intense immunoreactivity against acidic alpha-keratin; however, no basic alpha-keratin was detected immunohistologically. A pattern comparable to that observed in the spleen was detected in the second turtles liver and muscle tissue metastases. Discussion This study provides a catalogue of tissues and cells from bearded dragons and loggerhead sea turtles labelled by anti-alpha human keratin.